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How much is the annealing temperature? Let's explore the specific value together.

by:Kehua     2022-07-25
Annealing is a common process. So, do you know what the annealing temperature is? For this problem, many friends are also difficult to control in the actual operation process. Then, next, We will answer it for you right away. What is the general annealing temperature? 1. Annealing Kehua pointed out that annealing is a metal heat treatment process, which means that the metal is slowly heated to a certain temperature, kept for a sufficient time, and then cooled at a suitable speed. The purpose is to reduce hardness, improve machinability; eliminate residual stress, stabilize size, reduce deformation and crack tendency; refine grains, adjust organization, and eliminate organizational defects. To be precise, annealing is a heat treatment process for materials, including metallic materials and non-metallic materials. Moreover, the purpose of annealing of new materials is similar to that of traditional metal annealing. 2. Purpose of annealing 1. Reduce the hardness of steel and improve plasticity, so as to facilitate cutting and cold deformation processing. 2. Refine the grains, homogenize the structure and composition of the steel, improve the properties of the steel or prepare for the subsequent heat treatment. 3. Remove residual internal stress in steel to prevent deformation and cracking. What is the annealing temperature? 3. What is the annealing temperature? Kehua believes that the annealing temperature (Annealing Temperature) is the temperature parameter when the primer and the template are combined. When 50% of the primer and the complementary sequence appear as double-stranded DNA molecules, it is The more important factors affecting the specificity of PCR. Ideally, the annealing temperature is low enough to ensure efficient annealing of the primer to the target sequence, and high enough to reduce nonspecific binding. Reasonable annealing temperatures range from 55°C to 70°C. The annealing temperature is generally set to be 5°C lower than the Tm of the primer. What is the annealing temperature in general? You must have found the most reasonable temperature above, so that when you anneal the appliance, you finally know how to set the temperature. I hope the above content can solve your problem.
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